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#0183;#32;The tube was capped and shaken vertically for 5 min at 1500 strokes/min using an Automill automatic pulverizer (Tokken). After a brief centrifugation, the bullet was removed using tweezers and the suspension was filtered using a centrifugal filter unit fitted with a polyvinylidene fluoride microporous membrane (Ultrafree MC, Millipore, Billerica, MA).
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#0183;#32;The tube was capped and shaken vertically for 5 min at 1500 strokes/min with an automatic pulverizer (Automill, Tokken). A total of 12 tubes could be processed in parallel. After a brief centrifugation, the bullet was removed with tweezers and the tube centrifuged again for 2 min.
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#0183;#32;A stainlesssteel bullet (Tokken, Kashiwa, Japan), 20 L of 1 g/mL 3amino1phenylbutane in water and 230 L of water (for identification, no 3amino1phenylbutane and 250 L of water) were added. The tube was capped and shaken vertically for 5 min at 1500 strokes/min in an automatic pulverizer (Automill, Tokken).
#0183;#32;Seed samples were ground for 1 min at 1500 strokes per min with an automatic pulverizer (Automill TKAM5, Tokken Inc., Chiba, Japan). Genomic DNA was extracted from each ground sample (approximately 1 g) using the DNeasy Plant Maxi Kit (Qiagen , Hilden, Germany) according to the manufacturers instructions with the exception of the use of 300 L of elution volume without ethanol
Laboratory Pulverizer Ball MillSmall Planetary Ball Grinding Mill Machine Please notice that our price doesn''t include the ball jars and balls. Parameters: Small Lab Planetary Ball Mill line. Working Principle impact and friction force Item No. Compatible
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